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The study was conducted to identify optimal basal medium and plant growth regulators for multiplication of seedlings and rhizomes from asymbiotic germination of Cymbidium species which were native at a temperate zone. Seeds of Cymbidium kanran was well germinated in 3g/ℓ hyponex, 4g/ℓ peptone, 0.1㎎/ℓ NAA and 0.01㎎/ℓ kinetin containing medium. Multiplication of rhizomes of all the species which were tested in this experiment were highly stimulated in H3P4 medium containing 2.0㎎/ℓ NAA and 0.2㎎/ℓ kinetin. Growth of seedlings of Cymbidium kanran was more favorable in H3P4 medium with 1.0㎎/ℓ NAA and 3.0㎎/ℓ BA, and that of C. goeringii, with 1.0㎎/ℓ NAA and BA. On the other hand, seedlings of C. sinensis forma hakuran, C, faberi and C. ensifolium were grown well in the medium with 2.0㎎/ℓ NAA and 1.0㎎/ℓ BA. Shooting from rhizomes and growth of seedlings of six varieties and seven Fl hybrids were very good in H3P4 medium supplemented with 2.0㎎/ℓ NAA and 1.0㎎/ℓ BA. When these seedlings were transplanted into activated charcoal added medium, the seedlings were grown well to root.
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